세포 사멸 연구의 Trend, Ferroptosis에 주목하세요.
Dojindo는 Ferroptosis 관련 signaling pathway 및 분자 마커 관측에 적합한 다양한 제품을 보유하고 있습니다.
웅비에서 제공하는 제품 list를 함께 확인해보세요!
SnapShot: Ferroptosis
Kamyar Hadian¹ and Brent R. Stockwell²˒³
¹Assay Development and Screening Platform, Helmholtz Zentrum München, Neuherberg, Germany
²Department of Biological Sciences, Columbia University, New York, NY 10027, USA
³Department of Chemistry, Columbia University, New York, NY 10027, USA
https://doi.org/10.1016/j.cell.2020.04.039
- Labile Iron Pool (LIP)
▶ FerroOrange [Cat# F374] Intracellular Fe²⁺ 농도를 형광으로 측정
▶ Mito-FerroGreen [Cat# M489]
Mitochondrial Fe²⁺ 농도를 형광으로 측정
| Name | FerroOrange | Mito-FerroGreen |
| Catalog | F374 | M489 |
| Target | Ferrous Ion (Fe²⁺) | Ferrous Ion (Fe²⁺) |
| Localization | Intracellular | Mitochondria |
| Detection
(Ex/Em) |
Fluorescence
(543 nm/580 nm) |
Fluorescence
(505 nm/580 nm) |
| Instrument | Fluorescence Microscope | Fluorescence Microscope,
Microplate Reader |
| Sample | Live Cell | Live Cell |
- Lipid Peroxides & Oxidative Stress
► Liperfluo [Cat# L248] Intracellular Lipid Peroxides를 형광으로 측정
► MitoPeDPP [Cat# M466] Mitochondrial Lipid Peroxides를 형광으로 측정
by Prof. Koji Fukui, Molecular Cell Biology Laboratory, Shibaura Institute of Technology.
https://doi.Org/10.1080/10715762.2016.1227074
► MDA Assay Kit [Cat# M496]
Intracellular MDA(Malondialdehyde)를 형광으로 측정
– MDA Assay kit의 원리
– 경쟁사 제품 대비 간편한 실험 방법으로 시간 절약
► ROS Assay Kit – Photo-oxidation Resistant DCFH-DA- [Cat# R253]
Intracellular ROS를 형광으로 측정
– 빛에 노출되어도 결과를 신뢰할 수 있는 Photo-oxidation Resistant DCFH-DA
| Name | Liperfluo | MitoPeDPP | Lipid Peroxidation Probe -BDP 581/591 C11- | MDA Assay Kit | ROS Assay Kit -Photo-oxidation Resistant DCFH-DA- |
| Catalog | L248 | M466 | L267 | M496 | R253 |
| Target | Lipid
Peroxide |
Lipid Peroxidation | Lipid Peroxidation | Malondialdehyde | ROS (Reactive oxygen species) |
| Localization | Intracellular | Mitochondria | Intracellular | Intracellular | Intracellular |
| Detection
(Ex/Em) |
Fluorescence
(524 nm /535 nm) |
Fluorescence
(452 nm /470 nm) |
Fluorescence
(1. 488 nm /510–550 nm 2. 561 nm /600–630 nm) |
Fluorescence
(540 nm /590 nm) Colorimetric: 532 nm |
Fluorescence
(1. 488 nm /500-550 nm 2. 490-520nm /510-540 nm) |
| Instrument | Fluorescence Microscope,
FCM |
Fluorescence Microscope, FCM | Fluorescence Microscope, FCM, Microplate Reader | Microplate Reader | Fluorescence Microscope, FCM,
Microplate Reader |
| Sample | Live Cell | Live Cell | Live Cell | Cell, Tissue | Live Cell |
- GSSG/GSH Ratio
► GSSG/GSH Quantification Kit [Cat# G257]
Intracellular GSSG 및 GSH를 형광으로 측정
– GSSG/GSH Quantification Kit의 원리 및 reference 결과
– 간단한 실험 방법으로 1-2시간 내 결과 획득
- System xc– (xCT)
► Cystine Uptake Assay Kit [Cat# UP05]
Cystine Analog(CA)를 이용하여 cystine uptake 및 xCT activity를 형광으로 측정
– 전통적인 Radiolabeled [14C]에 비해 저렴하고 간편한 procedure
| Name | GSSG/GSH Quantification Kit | Glutamine Assay Kit-WST | Glutamate Assay Kit-WST | Cystine Uptake Assay Kit |
| Catalog | G257 | G268 | G269 | UP05 |
| Target | Glutathione (oxidized/reduced) | Glutamine | Glutamine | System xc–
(xCT) |
| Localization | Intracellular | Intracellular
/Extracellular |
Intracellular
/Extracellular |
Cell membrane |
| Detection
(Ex/Em) |
Colorimetric:
412 nm |
Colorimetric:
450 nm |
Colorimetric:
450 nm |
Fluorescence
(485 nm /535 nm) |
| Instrument | Microplate Reader | Microplate Reader | Microplate Reader | Microplate Reader |
| Sample | Cell, Tissue, Blood Plasma, Red Blood Cell | Cell, Culture Medium | Cell, Culture Medium | Live Cell |
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